Role of Hakai in m6A modification pathway in Drosophila

Abstract N6-methyladenosine (m 6 A), the most abundant internal modification in eukaryotic mRNA, is installed by a multi-component writer complex; however, exact roles of each component remain poorly understood. Here we show that potential E3 ubiquitin ligase Hakai colocalizes and interacts with other m A components, mutants exhibit typical pathway defects Drosophila , such as lowered levels aberrant Sxl alternative splicing, wing behavior defects. Hakai, Vir, Fl(2)d Flacc form stable complex, disruption either Vir or led to degradation three components. Furthermore, MeRIP-seq indicates effective mostly distributed 5’ UTRs contrast mammalian system. Interestingly, demonstrate deposited onto mRNA sex-specific fashion, which depends on writer. Together, our work not only advances understanding mechanism regulation but also provides insights into how cooperate control its own splicing.